CRISPR/CAS9-BASED DEPLETION OF 16S RIBOSOMAL RNA IMPROVES LIBRARY COMPLEXITY OF SINGLE-CELL RNA-SEQUENCING IN PLANARIANS

CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

Abstract Background Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material.To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key step during library preparation.However, some transcripts, such as mitochondrial genes,

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Improving the efficacy of ERP-based BCIs using different modalities of covert visuospatial attention and a genetic algorithm-based classifier.

We investigated whether the covert chains orienting of visuospatial attention can be effectively used in a brain-computer interface guided by event-related potentials.Three visual interfaces were tested: one interface that activated voluntary orienting of visuospatial attention and two interfaces that elicited automatic orienting of visuospatial at

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Finite Element Analysis to the Effect of Thermo-Mechanical Loads on Stress Distribution in Buried Polyethylene Gas Pipes Jointed by Electrofusion Sockets, Repaired by PE Patches

Polyethylene (PE) gas pipes can be jointed together by electrofusion PE fittings, which have sockets that are fused onto the pipe.Additionally, electrofused PE patches can be used to repair defected chains pipes.When these pipelines are buried under the ground, they can experience sever local stresses due to the presence of pipe joints, which is su

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