CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians
Abstract Background Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material.To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key step during library preparation.However, some transcripts, such as mitochondrial genes,